Application
Western Blot analysis of MKP-6 expression in transfected 293T cell line (20ug of whole cell ltsate) A: Non-transfected lysate. B: MKP-6 transfected lysate. 12% SDS PAGE diluted at 1:1000| Product Name | DUSP14 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DUSP14 (dual specificity phosphatase 14)In addition to antigen recognition by the T-cell receptor, T-cell activation requires a second signal from a costimulatory receptor, such as CD28 (MIM 186760), which interacts with B7-1 (CD80; MIM 112203) and B7-2 (CD86; MIM 601020) ligands on antigen-presenting cells. CD28 costimulation induces transcription of interleukin-2 (IL2; MIM 147680) and stabilizes newly synthesized IL2 through the activation of mitogen-activated protein kinases (MAPKs), such as ERK (e.g., MAP2K4; MIM 601335) and JNK (see MIM 601158), and the subsequent creation of AP1 transcription factor (see MIM 165160). DUSP14 is a negative regulator of CD28 signaling.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DUSP14. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MKP-L antibody MKP6 antibody DUSP14 antibody dual specificity protein phosphatase 14 antibody MAP kinase phosphatase 6 antibody MKP-6 antibody MKP-1-like protein tyrosine phosphatase antibody MKP-1 like protein tyrosine phosphatase antibody mitogen |
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| Molecular Weight(MW) | 22 kDa (note) |
Application
Western Blot analysis of MKP-6 expression in transfected 293T cell line (20ug of whole cell ltsate) A: Non-transfected lysate. B: MKP-6 transfected lysate. 12% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
