Application
Western blot detection of DUT-N in HeLa cell lysate at a dilution of 1:1000.| Product Name | DUT-N antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DUT - NDeoxyuridine triphosphate nucleotidohydrolase (dUTPase) is a ubiquitous enzyme that functions in the hydrolysis of dUTP to dUMP and pyrophosphate. Canman and co-workers have demonstrated that, in certain human tumor cell lines, increased levels of dUTPase are responsible for an increase in resistance to the cancer chemotherapeutic agent fluorodeoxyuridine (FUdR), a thymidine synthase inhibitor. Two distinct forms of dUTPase exist in humans. Cellular fractionation experiments suggest that the more abundant, lower mass form of dUTPase (DUT-N) localizes in the nucleus, while the higher mass form (DUT-M) is associated with the mitochondria. |
| Immunogen | Synthetic peptide, corresponding to amino acids 25-44 (NARGTAEGRSRGTLRARPAP) and 113-130 (QLRFARLSEHATAPTRGS) of Human DUT-N. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 601266 dUTPase DUT DUTN 1854 P33316 |
|---|
Application
Western blot detection of DUT-N in HeLa cell lysate at a dilution of 1:1000.| Application Notes | WB: Use at a dilution of 1/1000 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
