| Product Name | Dynamin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4E67] to Dynamin 1Dynamin is a 100 kDa GTP binding protein that was first identified as a protein able to bind microtubules. A mutation in the Drosophila gene shits, encoding a dynamin homologue, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles. Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles. Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this complex. To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific, Dynamin 2 is ubiquitously expressed and Dynamin 3 localizes to testis, brain and lung. |
| Immunogen | Recombinant fragment, (His)6 tagged, corresponding to amino acids 1-750 of Human Dynamin 1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IHCIPWB |
| IHC: Use at an assay dependent dilution. Staining of formaldehyde perfused and fixed rat brain shows synaptic immunofluorescence. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 mg/ml. Predicted molecular weight: 97 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRatBovine |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 1759 Dynamin 1 602377 Q05193 DNM1 DNM Dynamin1 |
|---|
| Application Notes | IHC: Use at an assay dependent dilution. Staining of formaldehyde perfused and fixed rat brain shows synaptic immunofluorescence. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 mg/ml. Predicted molecular weight: 97 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. |
| Storage Buffer | Preservative: None; Constituents: 0.1mM PMSF, PBS, 40% glycerol. |
