| Product Name | Dynamin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [0.T.178] to DynaminDynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at a concentration of 10 μg/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 0.2 μg/ml. Predicted molecular weight: 100 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | 1759 602377 Q05193 DNM1 Dynamin DNM |
|---|
| Application Notes | ICC: Use at a concentration of 10 μg/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 0.2 μg/ml. Predicted molecular weight: 100 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 40 % glycerol |
