Application
Flow cytometric Analysis of Hela cells, using anti-DYNC1LI1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DYNC1LI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DYNC1LI1.Application
Anti-DYNC1LI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with DYNC1LI1Application
HEK293T cells transfected with either RC222010 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DYNC1LI1 antibody, and then analyzed by flow cytometry.Application
Flow cytometric Analysis of Jurkat cells, using anti-DYNC1LI1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Product Name | DYNC1LI1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2E8] to DYNC1LI1Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores. |
| Immunogen | Full length human recombinant protein of human DYNC1LI1 (NP_057225) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DNCLI1 |
|---|
Application
Flow cytometric Analysis of Hela cells, using anti-DYNC1LI1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DYNC1LI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DYNC1LI1.
Application
Anti-DYNC1LI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with DYNC1LI1
Application
HEK293T cells transfected with either RC222010 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DYNC1LI1 antibody, and then analyzed by flow cytometry.
Application
Flow cytometric Analysis of Jurkat cells, using anti-DYNC1LI1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
