Mouse monoclonal [MANDRA1] to DystrophinThe C-terminal domain of the human dystrophin molecule (a. A. residues 3558-3684) is present in normal muscle tissue. It is also present in nearly all Becker muscular dystrophies, but is absent in cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx) (ref 1-3).
Immunogen
Fusion protein, corresponding to amino acids 3200-3684 of Human Dystrophin.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
ELISAICC/IFIHC-FWB
ELISA: Use at an assay dependant dilution. See Nyugen thi Man et al (1992) and Morris G.E et al (1993) ICC/IF: Use at a dilution of 1/100 for freshly dissected and frozen human or animal muscle tissue. IHC-Fr: Use at an assay dependant dilution. Immunohistochemical staining of muscle tissue results in a clear labeling confined to the periphery (plasma membrane) of normal striated muscle fibers. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.:
IHC-Fr analysis of human tongue tissue using Dystrophin antibody [MANDRA1] at a dilution of 1:100.
Application Notes
ELISA: Use at an assay dependant dilution. See Nyugen thi Man et al (1992) and Morris G.E et al (1993) ICC/IF: Use at a dilution of 1/100 for freshly dissected and frozen human or animal muscle tissue. IHC-Fr: Use at an assay dependant dilution. Immunohistochemical staining of muscle tissue results in a clear labeling confined to the periphery (plasma membrane) of normal striated muscle fibers. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.