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Western blotting: use at a dilution of 1/1000 - 1/3000. ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA). A 1:10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction. Appropriate specificity controls were run. Optimal dilutions / concentrations should be determined by the end user.:
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