Application
E2F1 antibody [N1N3] detects E2F1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E2F1 protein stained by E2F1 antibody [N1N3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Sample (30 µg of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
E2F1 antibody [N1N3] immunoprecipitates E2F1 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of E2F1 protein by 4 μg E2F1 antibody [N1N3] 10 % SDS-PAGE The immunoprecipitated E2F1 protein was detected by E2F1 antibody [N1N3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | E2F1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E2F1 (E2F transcription factor 1)The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, BovineRat (97%), Chicken (86%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | E2F-1 antibody RBAP1 antibody RBBP3 antibody RBP3 antibody E2F1 antibody retinoblastoma-binding protein 3 antibody RBAP-1 antibody PRB-binding protein E2F-1 antibody RBBP-3 antibody transcription factor E2F1 antibody PBR3 antibody retinoblastoma |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) |
| Cellular Localization | Nucleus |
Application
E2F1 antibody [N1N3] detects E2F1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E2F1 protein stained by E2F1 antibody [N1N3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Sample (30 µg of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
E2F1 antibody [N1N3] immunoprecipitates E2F1 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of E2F1 protein by 4 μg E2F1 antibody [N1N3] 10 % SDS-PAGE The immunoprecipitated E2F1 protein was detected by E2F1 antibody [N1N3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
