Application
Sample (30 µg of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | E2F3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E2F3 (E2F transcription factor 3)The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human E2F3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, BovineMouse (98%), Xenopus laevis (81%), Bovine (98%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686C18211 antibody E2F-3 antibody KIAA0075 antibody MGC104598 antibody E2F3 antibody transcription factor E2F3 antibody E2F transcription factor 3 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) (2) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data. |
Application
Sample (30 µg of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
