| Product Name | E2F3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PG30] to E2F3E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 3 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides. |
| Immunogen | Recombinant protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | PG30 |
| Host Species | Mouse |
| Tested Applications | GSAIPWB |
| GSA: Use at an assay dependent dilution. IP (denatured): Use at an assay dependent dilution. WB: Use at a concentration of 4 - 8 μg/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F3. Detects a band of approximately 57 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | KIAA0075 1871 E2F3 600427 E2F-3 O00716 MGC104598 DKFZp686C18211 E2F 3 |
|---|---|
| Target | E2F3 |
| Tissue Specificity | Does not detect the other members of the E2F family. |
| Cellular Localization | Nuclear |
| Application Notes | GSA: Use at an assay dependent dilution. IP (denatured): Use at an assay dependent dilution. WB: Use at a concentration of 4 - 8 μg/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F3. Detects a band of approximately 57 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH70 7.4 |
