Application
WB analysis of HepG2 cells using E2F4 antibody at 0.2-1μg/ml.Application
IHC-P analysis of human intestine tissue using E2F4 antibody at 4.0-8.0μg/ml.Application
IHC-P analysis of human stomach tissue using E2F4 antibody at 4.0-8.0μg/ml.| Product Name | E2F4 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E2F4The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to a C-terminal region of Human E2F4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | E2F4 |
|---|---|
| Target | E2F4 |
| Molecular Weight(MW) | 44 KDa (note) |
Application
WB analysis of HepG2 cells using E2F4 antibody at 0.2-1μg/ml.
Application
IHC-P analysis of human intestine tissue using E2F4 antibody at 4.0-8.0μg/ml.
Application
IHC-P analysis of human stomach tissue using E2F4 antibody at 4.0-8.0μg/ml.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
