Application
Antibody used in IHC on Human Intestine.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Stomach.| Product Name | E2F4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F4, E2F-4 |
| Product description | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.1) DuPree, E.L., et al., (2004) Cancer Res. 64 (13), 4390-4393. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| E2F4 antibody can be used for detection of E2F4 by ELISA at 1:312500. E2F4 antibody can be used for detection of E2F4 by western blot at 2.0/mL.: |
|
| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F4 |
|---|---|
| Alternative Names | E2F4 E2F-4 |
| Molecular Weight(MW) | 44 kDa |
Application
Antibody used in IHC on Human Intestine.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Stomach.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | E2F4 antibody can be used for detection of E2F4 by ELISA at 1:312500. E2F4 antibody can be used for detection of E2F4 by western blot at 2.0/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
