Application
E2F5 antibody detects E2F5 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of HA-human E2F5-transfected 293T cells 10 % SDS-PAGE E2F5 antibody dilution: 1:10000| Product Name | E2F5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E2F5 (E2F transcription factor 5, p130-binding)The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human E2F5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | E2F transcription factor 5 p130-binding E2F-5 |
|---|---|
| Target | E2F5 |
| Molecular Weight(MW) | 38 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
E2F5 antibody detects E2F5 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of HA-human E2F5-transfected 293T cells 10 % SDS-PAGE E2F5 antibody dilution: 1:10000| Positive Control | HA-human E2F5-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
