Application
Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE diluted at 1:500| Product Name | EB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to EB1 (microtubule-associated protein, RP/EB family, member 1)The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, BovineMouse (96%), Rat (91%), Xenopus laevis (81%), Dog (97%), Pig (97%), Chicken (85%), Rhesus Monkey (99%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EB1 antibody MGC117374 antibody MGC129946 antibody MAPRE1 antibody microtubule-associated protein RP/EB family member 1 antibody APC-binding protein EB1 antibody end-binding protein 1 antibody adenomatous polyposis coli-binding protein EB1 antibody |
|---|---|
| Target | EB1 |
| Molecular Weight(MW) | 30 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE diluted at 1:500| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
