Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000Application
Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 μg of EBF3 antibody [C1C3] . Western blot analysis was performed using EBF3 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
EBF3 antibody [C1C3] detects EBF3 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EBF3 protein stained by EBF3 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | EBF3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to EBF3 (early B-cell factor 3)Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EBF3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevisMouse (99%), Rat (99%), Xenopus laevis (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COE3 antibody O/E-2 antibody EBF3 antibody transcription factor COE3 antibody olf-1/EBF-like 2 antibody early B-cell factor 3 antibody |
|---|---|
| Target | EBF3 |
| Molecular Weight(MW) | 65 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000
Application
Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 μg of EBF3 antibody [C1C3] . Western blot analysis was performed using EBF3 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
EBF3 antibody [C1C3] detects EBF3 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EBF3 protein stained by EBF3 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
