Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:10000Application
EBP1 antibody immunoprecipitates EBP1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of EBP1 protein by 2 μg of EBP1 antibody 7.5% SDS-PAGE The immunoprecipitated EBP1 protein was detected by EBP1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Product Name | EBP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EBP1 (proliferation-associated 2G4, 38kDa)This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EBP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chimpanzee, BovineMouse (99%), Rat (99%), Xenopus laevis (92%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EBP1 antibody HG4-1 antibody p38-2G4 antibody PA2G4 antibody erbB3-binding protein 1 antibody proliferation-associated protein 2G4 antibody ErbB-3 binding protein 1 antibody ErbB3-binding protein Ebp1 antibody cell cycle protein p38-2G4 homolog ant |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:10000
Application
EBP1 antibody immunoprecipitates EBP1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of EBP1 protein by 2 μg of EBP1 antibody 7.5% SDS-PAGE The immunoprecipitated EBP1 protein was detected by EBP1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Positive Control | A431 , Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
