Application
Western Blot analysis of ECEL1 expression in transfected 293T cell line by ECEL1 polyclonal antibody. A: Non-transfected lysate. B: ECEL1 transfected lysate. 7.5% SDS PAGE diluted at 1:1000| Product Name | ECEL1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ECEL1 (endothelin converting enzyme-like 1)This gene encodes a member of the M13 family of endopeptidases. In general, M13 family members are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. This gene is expressed specifically in the central nervous system and its protein localizes predominately to the endoplasmic reticulum or, in trace amounts, to the cell surface. Disruption of this gene in mouse embryonic stem cells results in neonatal lethality due to respiratory failure shortly after birth. Based on the specific expression of this gene and the phenotype of the gene deficiency in mouse embryos, it is suggested that this protein plays a critical role in neural regulation of the respiratory system. This gene has multiple pseudogenes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ECEL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (95%), Rat (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DINE antibody ECEX antibody XCE antibody ECEL1 antibody X converting enzyme antibody endothelin-converting enzyme-like 1 antibody damage induced neuronal endopeptidase antibody endothelin converting enzyme-like 1 antibody |
|---|---|
| Target | ECEL1 |
| Molecular Weight(MW) | 88 kDa (note) (2) |
| Cellular Localization | Membrane |
Application
Western Blot analysis of ECEL1 expression in transfected 293T cell line by ECEL1 polyclonal antibody. A: Non-transfected lysate. B: ECEL1 transfected lysate. 7.5% SDS PAGE diluted at 1:1000| Positive Control | ECEL1 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
