Application
Western blot of Ecm29 in HeLa cells using Ecm29 antibody.Application
Immunofluorescent staining of Ecm29 in HeLa cells using Ecm29 antibody . Ecm29 (shown in red) is detected with secondary antibody goat anti-rabbit Alexa 568. Tubulin (shown in green) is detected with secondary antibody goat ant-mouse Alexa 488. (yellow is the overlap)| Product Name | Ecm29 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal Antibody to Ecm29Ecm29 appears to be one of three proteins which associate significantly with proteasomes. Ecm29 has been shown to enhance the stability of the proteasome. Ecm29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that Ecm29 tethers the regulatory complex to the proteasome. |
| Immunogen | GTX82906 immunogen corresponds to amino acid residues 1821-1839 of the C-terminus of human Ecm29. Synthetic peptide: R(1821) P E L Q E K A A L L K K T L E N L E(1839). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For IF: Use at a dilution of 1:200. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RP11-386D8.2 KIAA1962 RP11386D8.2 Ecm29 KIAA0368 Q5VYK3 23392 FLJ22036 |
|---|---|
| Target | Ecm29 |
Application
Western blot of Ecm29 in HeLa cells using Ecm29 antibody.
Application
Immunofluorescent staining of Ecm29 in HeLa cells using Ecm29 antibody . Ecm29 (shown in red) is detected with secondary antibody goat anti-rabbit Alexa 568. Tubulin (shown in green) is detected with secondary antibody goat ant-mouse Alexa 488. (yellow is the overlap)| Application Notes | For IF: Use at a dilution of 1:200. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Contains 0.05% sodium azide |
