Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:3000Application
Edc3 antibody [N2C2], Internal detects Edc3 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Edc3 antibody [N2C2], Internal dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Edc3 antibody at 1:250 dilution.Application
Edc3 antibody [N2C2], Internal detects EDC3 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Edc3 antibody [N2C2], Internal dilution: 1:1000| Product Name | Edc3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Edc3 (enhancer of mRNA decapping 3 homolog (S. cerevisiae))EDC3 is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Edc3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Chicken, Rhesus MonkeyXenopus laevis (83%), Chicken (92%), Rhesus Monkey (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21128 antibody FLJ31777 antibody LSM16 antibody YJDC antibody YJEFN2 antibody EDC3 antibody yjeF N-terminal domain-containing protein 2 antibody hYjeF_N2 antibody yjeF domain-containing protein 1 antibody yjeF_N2 antibody yjeF domain containin |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (4) |
| Cellular Localization | Cytoplasm , P-body |
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:3000
Application
Edc3 antibody [N2C2], Internal detects Edc3 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Edc3 antibody [N2C2], Internal dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Edc3 antibody at 1:250 dilution.
Application
Edc3 antibody [N2C2], Internal detects EDC3 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Edc3 antibody [N2C2], Internal dilution: 1:1000| Positive Control | A431 , NIH-3T3 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
