Application
EDC4 antibody detects EDC4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. EDC4 antibody dilution: 1:500.Application
EDC4 antibody detects EDC4 protein at P-body by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDC4 protein stained by EDC4 antibody diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]Application
Sample (30 ug of whole cell lysate) A: MCF-7 5% SDS PAGE diluted at 1:3000Application
Sample (50 ug of whole cell lysate) A: mouse brain 5% SDS PAGE diluted at 1:2000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EDC4 antibody at 1:100 dilution.| Product Name | EDC4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EDC4 (enhancer of mRNA decapping 4)In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro). |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human EDC4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Ge-1 antibody HEDLS antibody RCD-8 antibody EDC4 antibody autoantigen Ge-1 antibody enhancer of mRNA-decapping protein 4 antibody human enhancer of decapping large subunit antibody autoantigen RCD-8 antibody enhancer of mRNA decapping 4 antibody |
|---|---|
| Molecular Weight(MW) | 152 kDa (note) (2) |
| Cellular Localization | Cytoplasm , P-body , Nucleus |
Application
EDC4 antibody detects EDC4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. EDC4 antibody dilution: 1:500.
Application
EDC4 antibody detects EDC4 protein at P-body by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDC4 protein stained by EDC4 antibody diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Application
Sample (30 ug of whole cell lysate) A: MCF-7 5% SDS PAGE diluted at 1:3000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 5% SDS PAGE diluted at 1:2000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EDC4 antibody at 1:100 dilution.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
