Application
U87-MG whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EDD antibody diluted at 1:500.Application
EDD antibody detects EDD protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDD protein stained by EDD antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | EDD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EDD (ubiquitin protein ligase E3 component n-recognin 5)This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human EDD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Zebrafish, BovineMouse (98%), Zebrafish (83%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DD5 antibody E3 identified by differential display antibody E3 ubiquitin protein ligase HECT domain containing 1 antibody E3 ubiquitin-protein ligase UBR5 antibody E3 ubiquitin-protein ligase HECT domain-containing 1 antibody EDD antibody |
|---|---|
| Target | EDD |
| Molecular Weight(MW) | 309 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
U87-MG whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EDD antibody diluted at 1:500.
Application
EDD antibody detects EDD protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDD protein stained by EDD antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | U87-MG , U87-MG nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
