Application
Jurkat whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EDEM1 antibody diluted at 1:1000.| Product Name | EDEM1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EDEM1 (ER degradation enhancer, mannosidase alpha-like 1)Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It lacks mannosidase activity. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human EDEM1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (87%), Rat (87%), Rhesus Monkey (100%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EDEM ER degradation enhancer mannosidase alpha-like 1 |
|---|---|
| Target | EDEM1 |
| Molecular Weight(MW) | 74 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane |
Application
Jurkat whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EDEM1 antibody diluted at 1:1000.| Positive Control | Jurkat , Jurkat membrane extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
