Application
Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1 antibody at 1:500 dilution.Application
EDG1 antibody [C2C3], C-term detects EDG1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDG1 protein stained by EDG1 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample(30 μg of whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:1000Application
EDG1 antibody [C2C3], C-term detects S1PR1 protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 10 % SDS-PAGE EDG1 antibody [C2C3], C-term dilution: 1:1000| Product Name | EDG1 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EDG1 (sphingosine-1-phosphate receptor 1)The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDG1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Xenopus Tropicalis, Rabbit, BovineRat (93%), Zebrafish (92%), Xenopus laevis (93%), Xenopus Tropicalis (93%), Rabbit (93%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CHEDG1 antibody D1S3362 antibody ECGF1 antibody EDG-1 antibody EDG1 antibody FLJ58121 antibody S1P1 antibody S1PR1 antibody sphingosine 1-phosphate receptor Edg-1 antibody endothelial differentiation sphingolipid G-protein-coupled receptor 1 ant |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (2) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1 antibody at 1:500 dilution.
Application
EDG1 antibody [C2C3], C-term detects EDG1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDG1 protein stained by EDG1 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample(30 μg of whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:1000
Application
EDG1 antibody [C2C3], C-term detects S1PR1 protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 10 % SDS-PAGE EDG1 antibody [C2C3], C-term dilution: 1:1000| Positive Control | HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
