Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EEA1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. EEA1 antibody [C3], C-term dilution: 1:500.Application
EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 5% SDS-PAGE EEA1 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EEA1 antibody [C3], C-term dilution: 1:500.Application
EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract B. 30 µg A375 whole cell lysate/extract 5% SDS-PAGE EEA1 antibody [C3], C-term dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. EEA1 antibody [C3], C-term dilution: 1:500.| Product Name | EEA1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EEA1 (early endosome antigen 1)Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MST105 antibody MSTP105 antibody ZFYVE2 antibody EEA1 antibody zinc finger FYVE domain-containing protein 2 antibody early endosome-associated protein antibody endosome-associated protein p162 antibody early endosome antigen 1 antibody early endoso |
|---|---|
| Molecular Weight(MW) | 162 kDa (note) |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EEA1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. EEA1 antibody [C3], C-term dilution: 1:500.
Application
EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 5% SDS-PAGE EEA1 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EEA1 antibody [C3], C-term dilution: 1:500.
Application
EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract B. 30 µg A375 whole cell lysate/extract 5% SDS-PAGE EEA1 antibody [C3], C-term dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. EEA1 antibody [C3], C-term dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
