Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 C:HeLa S3 12% SDS PAGE diluted at 1:1000Application
EEF1B2 antibody [N1C3] detects EEF1B2 protein at cytoplasm on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. EEF1B2 antibody [N1C3] diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EF-1-beta2 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EEF1B2 antibody at 1:250 dilution.| Product Name | EEF1B2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EEF1B2 (eukaryotic translation elongation factor 1 beta 2)This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EEF1B2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Rabbit, Chicken, Rhesus Monkey, BovineRat (94%), Xenopus laevis (85%), Pig (98%), Rabbit (99%), Chicken (100%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EEF1B antibody EEF1B1 antibody EF1B antibody EEF1B2 antibody elongation factor 1-beta antibody eukaryotic translation elongation factor 1 beta 1 antibody EF-1-beta antibody eukaryotic translation elongation factor 1 beta 2 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (3) |
Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 C:HeLa S3 12% SDS PAGE diluted at 1:1000
Application
EEF1B2 antibody [N1C3] detects EEF1B2 protein at cytoplasm on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. EEF1B2 antibody [N1C3] diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EF-1-beta2 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EEF1B2 antibody at 1:250 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
