Application
Sample(30 μg of whole cell lysate) A:A431 12% SDS PAGE diluted at 1:1000Application
EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. EEF1E1 antibody [N1C3] diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EEF1E1 antibody at 1:200 dilution.Application
EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500.| Product Name | EEF1E1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EEF1E1 (eukaryotic translation elongation factor 1 epsilon 1)This gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EEF1E1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (87%), Pig (92%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AIMP3 antibody P18 antibody EEF1E1 antibody aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 antibody ARS-interacting multifunctional protein 3 antibody p18 component of aminoacyl-tRNA synthetase complex antibody multisynthase |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample(30 μg of whole cell lysate) A:A431 12% SDS PAGE diluted at 1:1000
Application
EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. EEF1E1 antibody [N1C3] diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EEF1E1 antibody at 1:200 dilution.
Application
EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500.| Positive Control | A431 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
