Application
eEF2 antibody detects eEF2 protein at cytosol on human colon by immunohistochemical analysis. Sample: Paraffin-embedded colon. eEF2 antibody dilution: 1:500.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal diluted at 1:1000.Application
eEF2 antibody detects eEF2 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. eEF2 antibody dilution: 1:500.Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal diluted at 1:500.Application
Sample(30 μg of whole cell lysate) A:293T B:A431 7.5% SDS PAGE diluted at 1:1500Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal diluted at 1:500.| Product Name | eEF2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EEF2 (eukaryotic translation elongation factor 2)This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human eEF2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, Rhesus Monkey, BovineChicken (95%), Rhesus Monkey (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EEF-2 antibody EF2 antibody EEF2 antibody polypeptidyl-tRNA translocase antibody elongation factor 2 antibody eukaryotic translation elongation factor 2 antibody |
|---|---|
| Molecular Weight(MW) | 95 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
eEF2 antibody detects eEF2 protein at cytosol on human colon by immunohistochemical analysis. Sample: Paraffin-embedded colon. eEF2 antibody dilution: 1:500.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal diluted at 1:1000.
Application
eEF2 antibody detects eEF2 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. eEF2 antibody dilution: 1:500.
Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal diluted at 1:500.
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 7.5% SDS PAGE diluted at 1:1500
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal diluted at 1:500.| Positive Control | 293T , A431 , Neuro2A , NIH-3T3 , Neuro 2A , Raw264.7 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
