Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using eEF-2K antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:5000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using eEF-2K antibody at 1:200 dilution.| Product Name | eEF-2K antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EEF2K (eukaryotic elongation factor-2 kinase)This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human eEF-2K. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSU93850 antibody MGC45041 antibody eEF-2K antibody EEF2K antibody eEF-2 kinase antibody eukaroytic elongation factor 2 kinase antibody calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody calmodulin-dependent protein kinase I |
|---|---|
| Molecular Weight(MW) | 82 kDa (note) |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using eEF-2K antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:5000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using eEF-2K antibody at 1:200 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
