Application
Sample(30 μg of whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:1000| Product Name | EF-1alpha1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to EF-1 alpha1 (eukaryotic translation elongation factor 1 alpha 1)This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EF-1alpha1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Cat, Pig, Rabbit, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Rat (100%), Xenopus laevis (98%), Dog (100%), Cat (100%), Pig (100%), Rabbit (100%), Chicken (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCS-3 antibody CCS3 antibody EE1A1 antibody EEF-1 antibody EEF1A antibody EF-Tu antibody EF1A antibody FLJ25721 antibody GRAF-1EF antibody HNGC:16303 antibody LENG7 antibody MGC102687 antibody MGC131894 antibody MGC16224 antibody PTI1 antibod |
|---|---|
| Target | EF-1 alpha1 |
| Molecular Weight(MW) | 50 kDa (note) |
| Tissue Specificity | Based on a BLAST result, it may also cross with human eEF1A2. |
| Cellular Localization | Cytoplasm |
Application
Sample(30 μg of whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
