Application
Immunocytochemistry/Immunofluorescence: Eg5 Antibody [ - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.Application
Western Blot: Eg5 Antibody [ - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF11.| Product Name | Eg5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Eg5Eg5 is a mitotic motor protein required for bipolar spindle formation. The mitotic spindle is crucial for chromosome segregation during cell division. |
| Immunogen | Produced by immunization of rabbits with a recombinant segment of the coiled-coil domain of the human protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| This antibody may be used for Western blotting, immunoprecipitation, and immunofluorescence. A band at ~125 kDa can be detected by Western blot. Other applications have not been tested.Suggested working dilutions: *Western Blot - 1:1,000Immunoprecipitation - 1:1,000Immunofluorescence - 1:1,000*The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | HumanMammalian species |
| Concentration | 1mg/ml |
| Gene Synonyms | KIF11 Eg5 3832 KNSL1 148760 P52732 HKSP TRIP5 |
|---|
Application
Immunocytochemistry/Immunofluorescence: Eg5 Antibody [ - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.
Application
Western Blot: Eg5 Antibody [ - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF11.| Application Notes | This antibody may be used for Western blotting, immunoprecipitation, and immunofluorescence. A band at ~125 kDa can be detected by Western blot. Other applications have not been tested.Suggested working dilutions: *Western Blot - 1:1,000Immunoprecipitation - 1:1,000Immunofluorescence - 1:1,000*The investigator should determine the optimal working dilution for a specific application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.1 ml of whole rabbit antisera. |
