Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of KIF11 Antibody in CHO cell line lysates (35ug/lane)| Product Name | KIF11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5, KIF11, EG5, KNSL1, TRIP5 |
| Product description | KIF11 belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.1) Tcherniuk,S., et.al., Biochem. Pharmacol. 79 (6), 864-872 (2010) |
| Immunogen | This KIF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human KIF11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HamsterHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIF11 |
|---|---|
| Alternative Names | Kinesin-like protein KIF11 Kinesin-like protein 1 Kinesin-like spindle protein HKSP Kinesin-related motor protein Eg5 Thyroid receptor-interacting protein 5 TR-interacting protein 5 TRIP-5 KIF11 EG5 KNSL1 TRIP5 |
| Molecular Weight(MW) | 119 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of KIF11 Antibody in CHO cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
