Application
Sample (30 ug of whole cell lysate) A: H1299 B: HeLaS3 7.5% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Eg5 antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Eg5 antibody at 1:200 dilution.| Product Name | Eg5 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Eg5 (kinesin family member 11)This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Eg5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EG5 antibody HKSP antibody KNSL1 antibody TRIP5 antibody KIF11 antibody kinesin-like protein 1 antibody TRIP-5 antibody kinesin-related motor protein Eg5 antibody kinesin-like protein KIF11 antibody thyroid receptor-interacting protein 5 antibody |
|---|---|
| Molecular Weight(MW) | 119 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: H1299 B: HeLaS3 7.5% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Eg5 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Eg5 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
