Application
Western blot analysis using EGF mAb against EGF-hIgGFc transfected HEK293 cell lysate.Application
Immunofluorescence analysis of NIH-3T3 cells using EGF mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | EGF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4E11] to EGFThis gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| Immunogen | The immunogen corresponding to human EGF expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4E11 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HOMG4 URG |
|---|---|
| Target | EGF |
| Molecular Weight(MW) | 134 kDa (note) |
Application
Western blot analysis using EGF mAb against EGF-hIgGFc transfected HEK293 cell lysate.
Application
Immunofluorescence analysis of NIH-3T3 cells using EGF mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
