Application
Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using EGF antibody at 1:250 dilution.| Product Name | EGF antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EGF (epidermal growth factor (beta-urogastrone))Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human EGF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HOMG4 antibody URG antibody EGF antibody pro-epidermal growth factor antibody beta-urogastrone antibody epidermal growth factor antibody |
|---|---|
| Molecular Weight(MW) | 134 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using EGF antibody at 1:250 dilution.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
