Application
WB analysis of human EGFR mutant recombinant protein using EGFR mutant antibody [5G9B5].Application
IHC-P analysis of rectum cancer tissues using EGFR mutant antibody [5G9B5].Application
ELISA analysis of control antigen and antigen using EGFR mutant antibody [5G9B5].Application
IHC-P analysis of cervical cancer tissues using EGFR mutant antibody [5G9B5].Application
FACS analysis of HepG2 cells using EGFR mutant antibody [5G9B5] (green) and negative control (purple).Application
WB analysis of (1) SPC-A-1, (2) A549, (3) HepG2 and (4) MCF-7 cell lysates using EGFR mutant antibody [5G9B5].Application
WB analysis of lysates from (1) HEK293 and (2) EGFR mutant-hIgGFc transfected HEK293 cells using EGFR mutant antibody [5G9B5].| Product Name | EGFR (mutant) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [5G9B5] to EGFR mutantThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Immunogen | The immunogen corresponding to a region within amino acids 693 and 893 of human EGFR mutant expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5G9B5 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-PWB |
| Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:2000.
For ELISA: Use at a dilution of 1:10000.
For IHC-P: Use at a dilution of 1:200-1:1000.
For FACS: Use at a dilution of 1:200-1:400.
Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | ERBB MENA HER1 ERBB1 EGFR PIG61 |
|---|---|
| Target | EGFR mutant |
| Molecular Weight(MW) | 175 kDa (note) |
Application
WB analysis of human EGFR mutant recombinant protein using EGFR mutant antibody [5G9B5].
Application
IHC-P analysis of rectum cancer tissues using EGFR mutant antibody [5G9B5].
Application
ELISA analysis of control antigen and antigen using EGFR mutant antibody [5G9B5].
Application
IHC-P analysis of cervical cancer tissues using EGFR mutant antibody [5G9B5].
Application
FACS analysis of HepG2 cells using EGFR mutant antibody [5G9B5] (green) and negative control (purple).
Application
WB analysis of (1) SPC-A-1, (2) A549, (3) HepG2 and (4) MCF-7 cell lysates using EGFR mutant antibody [5G9B5].
Application
WB analysis of lysates from (1) HEK293 and (2) EGFR mutant-hIgGFc transfected HEK293 cells using EGFR mutant antibody [5G9B5].| Application Notes | Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:2000.
For ELISA: Use at a dilution of 1:10000.
For IHC-P: Use at a dilution of 1:200-1:1000.
For FACS: Use at a dilution of 1:200-1:400.
Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
