Application
Untreated (–) and treated (+) A431 whole cell extracts (20 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Phospho-EGF Receptor (Tyr1068) antibody diluted at 1:2000.| Product Name | EGFR (phospho Tyr1068) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EGFR (phospho Tyr1068) (epidermal growth factor receptor)The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EGFR (phospho Tyr1068). The exact sequence is proprietary. |
| Modification | p-Tyr1068 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | RatRat (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | EGFR (phospho Tyr1068) |
|---|---|
| Molecular Weight(MW) | 134 kDa (note) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted |
Application
Untreated (–) and treated (+) A431 whole cell extracts (20 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Phospho-EGF Receptor (Tyr1068) antibody diluted at 1:2000.| Positive Control | A431 (starvation for 24 hr) , A431 (starvation 24 hr, and then 100 ng/ml EGF treatment for 15 min) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
