Application
ApplicationApplication
Detection of EGFR by IHC in Human Lung Squamous Cell Carcinoma| Product Name | EGFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [111.6] to Epidermal Growth Factor Receptor (EGFR)This antibody is specific to 170 kD known as first member (EGF-Receptor) of type I Family of growth factor receptors. Its epitope maps in the extracellular domain of EGFR. Overexpression of EGFR has been reported in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. EGFR is over expressed in squamous cell carcinoma. |
| Immunogen | Extracellular domain of human recombinant EGFR protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 111.6 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature or overnight at 40C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 370C. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERBB1 131550 1956 EGFR ERBB mENA P00533 |
|---|---|
| Target | EGFR |
| Cellular Localization | Cell membrane |
Application
Application
Application
Detection of EGFR by IHC in Human Lung Squamous Cell Carcinoma| Positive Control | Placenta |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature or overnight at 40C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 370C. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS, containing 1% BSA and 0.05% sodium azide |
