| Product Name | EGFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [199.12] to EGFREGFR is the prototype member of the type 1 receptor tyrosine kinases. EGFR is encoded by the cellular oncogene c-erbB-1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. EGFR over-expression in tumors indicates poor prognosis. EGFR over-expression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and stomach, and in squamous cell carcinoma. |
| Immunogen | The extracellular domain of human recombinant EGFR protein, purified. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 199.12 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects bands of approximately 170 + 145 kDa corresponding to the intact and vIII variants of EGFR. This antibody is suitable for use in immunoprecipitation and Western blot analysis of the extracellular domain of EGFR. This antibody does not inhibit EGF binding to its receptor. Not recommended for use as the coating antibody in ELISA due to the presence of BSA. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | ERBB1 131550 1956 EGFR ERBB mENA P00533 |
|---|---|
| Target | EGFR |
| Tissue Specificity | This antibody binds to the intact and vIII variant of EGFR. This antibody shows no cross reactivity with c-erbB-2, c-erbB-3, or c-erbB-4. |
| Cellular Localization | Cell Membrane and Secreted |
| Positive Control | A431 cells. |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects bands of approximately 170 + 145 kDa corresponding to the intact and vIII variants of EGFR. This antibody is suitable for use in immunoprecipitation and Western blot analysis of the extracellular domain of EGFR. This antibody does not inhibit EGF binding to its receptor. Not recommended for use as the coating antibody in ELISA due to the presence of BSA. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
