Application
Lung carcinoma labeled with Monoclonal Anti-Epidermal Growth Factor ReceptorApplication
Photomicrograph of a mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431). Cells were grown on coverslips, fixed and stained with Monoclonal Anti-EGF Receptor, clone no. 29.1 – green fluorescence; and Hoechst No. 33258 – blue nuclear stain. Coverslips were photographed on a NIKON Microphot-FXA| Product Name | EGFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [29.1] to EGFRThe receptor for Epidermal Growth Factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. It is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus. EGF-R has anextracellular region which binds EGF and consequently mediates the initial response of cells to EGF and an intra- cellular region which posseses the tyrosine kinase activity. As a result of EGF binding to its specific receptor, there is increased DNA synthesis as well as other events such as cell proliferation, differentiationand repair of damaged epithelial tissue. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R). It is hypothesized that an inappropriate activation of the human erb-B gene either by truncation or overexpression plays a role in the development of the malignancy. This hypothesis is supported by studies which have shown an increased number of EGF-R in various malignant tumors. High levels of EGF-R have been identified in sarcomas, gliomas, gynecological, breast, bladder and lungtumors. |
| Immunogen | Tissue / cell preparation (Human A431 cell line). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 29.1 |
| Host Species | Mouse |
| Tested Applications | PurifyELISAICC/IFIHCIPWB |
| AP: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/10000. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/3000. Detects a band of approximately 170 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogPigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERBB1 131550 1956 EGFR ERBB mENA P00533 |
|---|---|
| Target | EGFR |
| Tissue Specificity | Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding. |
| Cellular Localization | Cell Membrane and Secreted |
Application
Lung carcinoma labeled with Monoclonal Anti-Epidermal Growth Factor Receptor
Application
Photomicrograph of a mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431). Cells were grown on coverslips, fixed and stained with Monoclonal Anti-EGF Receptor, clone no. 29.1 – green fluorescence; and Hoechst No. 33258 – blue nuclear stain. Coverslips were photographed on a NIKON Microphot-FXA| Positive Control | A431 whole cell extract |
|---|---|
| Application Notes | AP: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/10000. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/3000. Detects a band of approximately 170 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide |
