| Product Name | EGFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [Cocktail R19 / 48 ( 111.6 + 199.12 + F4 + 11E8 )] to EGFREGFR is the prototype member of the type 1 receptor tyrosine kinases. EGFR is encoded by the cellular oncogene c-erbB-1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. EGFR over-expression in tumors indicates poor prognosis. EGFR over-expression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. |
| Immunogen | Purified extracellular and cytoplasmic domains of human recombinant EGFR protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | Cocktail R19/48 (111.6 + 199.12 + F4 + 11E8) |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 μg/ml. Detects bands of approximately 170 + 145 kDa corresponding to the intact and vIII variants of EGFR. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | ERBB1 131550 1956 EGFR ERBB mENA P00533 |
|---|---|
| Target | EGFR |
| Cellular Localization | Cell Membrane and Secreted |
| Positive Control | A431 cells |
|---|---|
| Application Notes | IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 μg/ml. Detects bands of approximately 170 + 145 kDa corresponding to the intact and vIII variants of EGFR. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2 to 8ºC. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
