Application
Frozen section of squamous cell carcinoma (vulva) stained with Monoclonal Anti-EGF Receptor, clone no. F4 using the APAAP method| Product Name | EGFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [F4] to EGFRThe receptor for Epidermal Growth Factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. It is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus. EGF-R has anextracellular region which binds EGF and consequently mediates the initial response of cells to EGF and an intra-cellular region which posseses the tyrosine kinase activity. As a result of EGF binding to its specific receptor, there is increased DNA synthesis as well as other events including cell proliferation, differentiation and repair of damaged epithelial tissue. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic and transmembrane part of the EGF-R (truncatedEGF-R). It is hypothesized that an inappropriate activation of the human erb-B gene either by truncation or overexpression plays a role in the development of the malignancy. This hypothesis is supported by studies, which have shown an increased number of EGF-R in various malignant tumors. High levels of EGF-R have been identified in sarcomas, gliomas, gynecological, breast, bladder, and lung tumors. |
| Immunogen | synthetically produced residues 985-996 of the human EGF receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | F4 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-PIPWB |
| ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 170 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERBB1 131550 1956 EGFR ERBB mENA P00533 |
|---|---|
| Target | EGFR |
| Cellular Localization | Cell Membrane and Secreted |
Application
Frozen section of squamous cell carcinoma (vulva) stained with Monoclonal Anti-EGF Receptor, clone no. F4 using the APAAP method| Positive Control | A431 whole cell lysate |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 170 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide |
