Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EHF antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using EHF antibody at 1:500 dilution.| Product Name | EHF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EHF (ETS homologous factor)This gene encodes a protein that belongs to an ETS transcription factor subfamily characterized by epithelial-specific expression (ESEs). The encoded protein acts as a transcriptional repressor and may be associated with asthma susceptibility. This protein may be involved in epithelial differentiation and carcinogenesis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EHF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, Chimpanzee, BovineRat (92%), Chicken (80%), Chimpanzee (99%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ESE3 antibody ESEJ antibody EHF antibody ETS domain-containing transcription factor antibody ETS homologous factor antibody hEHF antibody ESE3 transcription factor antibody epithelium-specific Ets transcription factor 3 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EHF antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using EHF antibody at 1:500 dilution.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
