Application
EIF1AY antibody detects EIF1AY protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 15 % SDS-PAGE EIF1AY antibody dilution: 1:1000Application
EIF1AY antibody detects EIF1AY protein at Golgi apparatus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold Methanol for 5 min. Green: EIF1AY protein stained by EIF1AY antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 ug of whole cell lysate) A: Raji 15% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using EIF1AY antibody at 1:500 dilution.| Product Name | EIF1AY antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF1AY (eukaryotic translation initiation factor 1A, Y-linked)This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF1AY. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Pig, Chicken, Rhesus Monkey, Chimpanzee, BovinePig (99%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF1AY antibody eukaryotic translation initiation factor 4C antibody eukaryotic translation initiation factor 1A Y chromosome antibody eukaryotic translation initiation factor 1A Y-chromosomal antibody eIF-1A Y isoform antibody eIF-4C antibody euka |
|---|---|
| Molecular Weight(MW) | 16 kDa (note) (3) |
Application
EIF1AY antibody detects EIF1AY protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 15 % SDS-PAGE EIF1AY antibody dilution: 1:1000
Application
EIF1AY antibody detects EIF1AY protein at Golgi apparatus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold Methanol for 5 min. Green: EIF1AY protein stained by EIF1AY antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 ug of whole cell lysate) A: Raji 15% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using EIF1AY antibody at 1:500 dilution.| Positive Control | Raji , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
