Application
Immunofluorescence staining of methanol-fixed Hela cells using eIF2 alpha (phospho-Ser49) Antibody .Application
Western blot analysis of extracts from Hela cells untreated or treated with starvation using eIF2a(phospho-Ser49) AntibodyApplication
IHC-P analysis of human lung carcinoma tissue using eIF2 alpha (phospho Ser49) antibody.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2 alpha (Phospho-Ser49) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | eIF2 alpha (phospho Ser49) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eIF2 alpha (Phospho Ser49)Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| Immunogen | Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2 alpha . |
| Modification | p-Ser49 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EIF-2 EIF-2A EIF-2alpha EIF2 EIF2A EIF2S1 eukaryotic translation initiation factor 2 subunit 1 eukaryotic translation initiation factor 2 subunit alpha eIF-2-alpha eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa |
|---|---|
| Target | eIF2 alpha (Phospho Ser49) |
| Molecular Weight(MW) | 38 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using eIF2 alpha (phospho-Ser49) Antibody .
Application
Western blot analysis of extracts from Hela cells untreated or treated with starvation using eIF2a(phospho-Ser49) Antibody
Application
IHC-P analysis of human lung carcinoma tissue using eIF2 alpha (phospho Ser49) antibody.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2 alpha (Phospho-Ser49) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
