Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:2000Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using eIF2 alpha antibody at 1:100 dilution.| Product Name | eIF2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eIF2 alpha (eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa)The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Xenopus laevis (94%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF-2 antibody EIF-2A antibody EIF-2alpha antibody EIF2 antibody EIF2A antibody EIF2S1 antibody eukaryotic translation initiation factor 2 subunit 1 antibody eukaryotic translation initiation factor 2 subunit alpha antibody eIF-2-alpha anti |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:2000
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using eIF2 alpha antibody at 1:100 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
