Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using eIF2 alpha antibody at 1:200 dilution.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] diluted at 1:5000.| Product Name | eIF2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eIF2 alpha (eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa)The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, Rhesus Monkey, BovineXenopus laevis (92%), Chicken (89%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF-2 antibody EIF-2A antibody EIF-2alpha antibody EIF2 antibody EIF2A antibody EIF2S1 antibody eukaryotic translation initiation factor 2 subunit 1 antibody eukaryotic translation initiation factor 2 subunit alpha antibody eIF-2-alpha anti |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using eIF2 alpha antibody at 1:200 dilution.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] diluted at 1:5000.| Positive Control | H1299 , Raji , *rat neuron , *HeLa , *N2a |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
