Application
Western blot analysis of extracts from C2C12 cells untreated or treated with PMA using eIF2b (phospho-Ser2) Antibody| Product Name | eIF2 beta (phospho Ser2) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eIF2 beta (Phospho Ser2)eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| Immunogen | Peptide sequence around phosphorylation site of serine 2 (M-S(p)-G-D-E) derived from eIF2 beta . |
| Modification | p-Ser2 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686L18198 EIF2 EIF2B EIF2beta MGC8508 EIF2S2 eukaryotic translation initiation factor 2 subunit beta eIF-2-beta protein phosphatase 1 regulatory subunit 67 eukaryotic initiation factor 2-beta eukaryotic translation initiation factor 2 beta |
|---|---|
| Target | eIF2 beta (Phospho Ser2) |
| Molecular Weight(MW) | 38 (note) |
Application
Western blot analysis of extracts from C2C12 cells untreated or treated with PMA using eIF2b (phospho-Ser2) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
