Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using HRI antibody at 1:200 dilution.Application
EIF2AK1 antibody detects EIF2AK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human MCF7 xenograft. EIF2AK1 antibody diluted at 1:500.Application
EIF2AK1 antibody detects EIF2AK1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE EIF2AK1 antibody dilution: 1:1000| Product Name | EIF2AK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF2AK1 (eukaryotic translation initiation factor 2-alpha kinase 1)The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF2AK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rabbit, BovineMouse (87%), Rat (87%), Dog (88%), Rabbit (83%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HCR antibody HRI antibody KIAA1369 antibody EIF2AK1 antibody heme sensitive initiation factor 2a kinase antibody heme-regulated initiation factor 2-alpha kinase antibody heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody heme-co |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (2) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using HRI antibody at 1:200 dilution.
Application
EIF2AK1 antibody detects EIF2AK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human MCF7 xenograft. EIF2AK1 antibody diluted at 1:500.
Application
EIF2AK1 antibody detects EIF2AK1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE EIF2AK1 antibody dilution: 1:1000| Positive Control | HeLa , A375 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
