Application
ICC/IF analysis of COS7 cells with (right) or withour (left) eIF2B3 antibody [1H3] at a dilution of 1:50 (green). F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.Application
WB analysis of various cell lysate (80μg per lane) using eIF2B3 antibody [1H3] at a dilution of 1:1000.Application
ICC/IF analysis of HeLa cells with (right) or withour (left) eIF2B3 antibody [1H3] at a dilution of 1:50 (green). F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.Application
IP analysis was performed with U2OS lysate and eIF2B3 antibody [1H3]. The precipitate was detected by the same antibody with EasyBlot as secondary antibody.Application
IHC-P analysis of human breast tissue with (right) or without (left) eIF2B3 antibody [1H3] at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.Application
IHC-P analysis of human uterus tissue with (right) or without (left) eIF2B3 antibody [1H3] at a dilution of 1:100. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.| Product Name | eIF2B3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1H3] to eIF2B3The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Immunogen | Full length human eIF2B3 produced in HEK293T cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H3 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | EIF2Bgamma EIF2B3 eukaryotic translation initiation factor 2B subunit 3 gamma 58kDa EIF-2B |
|---|---|
| Target | eIF2B3 |
Application
ICC/IF analysis of COS7 cells with (right) or withour (left) eIF2B3 antibody [1H3] at a dilution of 1:50 (green). F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Application
WB analysis of various cell lysate (80μg per lane) using eIF2B3 antibody [1H3] at a dilution of 1:1000.
Application
ICC/IF analysis of HeLa cells with (right) or withour (left) eIF2B3 antibody [1H3] at a dilution of 1:50 (green). F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Application
IP analysis was performed with U2OS lysate and eIF2B3 antibody [1H3]. The precipitate was detected by the same antibody with EasyBlot as secondary antibody.
Application
IHC-P analysis of human breast tissue with (right) or without (left) eIF2B3 antibody [1H3] at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Application
IHC-P analysis of human uterus tissue with (right) or without (left) eIF2B3 antibody [1H3] at a dilution of 1:100. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C |
| Storage Buffer | PBS with 1mg/ml BSA, 30% glycerol and 0.05% sodium azide |
