Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using EIF3 beta antibody at 1:200 dilution.| Product Name | EIF3 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF3 beta (eukaryotic translation initiation factor 3, subunit I)Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF3 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Rabbit, Chicken, BovineMouse (98%), Rat (98%), Xenopus laevis (88%), Pig (98%), Rabbit (96%), Chicken (92%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF3S2 antibody, PRO2242 antibody, TRIP-1 antibody, TRIP1 antibody, eIF3-beta antibody, eIF3-p36 antibody, EIF3I antibody, predicted protein of HQ2242 antibody, TGF-beta receptor-interacting protein 1 antibody, TGFbeta receptor-interacting protein 1 antibo |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using EIF3 beta antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
